The cell process that I hypothesize that will be affected by my mutations would be transcription regulation What is the gene? Gene type: protein codingGene description: putative DNA-binding transcriptional regulatorRegulates the transcription of the frlr operon. Involved in the utilization of fructoselysine and psicoselysine.Pathway: fructoselysine degradationThis protein regulates the pathway fructoselysine degradation. Part of Carbohydrate metabolism.DNA-Binding-Region is H-T-H motif.Conserved-Region is HTH gntR-type.The gntR-type HTH domain is a DNA-binding – winged helix-turn-helix (wHTH) domain of about 60-70 residues present in transcriptional regulators of the gntR family.In proteins, the helix-turn-helix (HTH) is a major structural motif which can bind DNA.HTH can be seen in proteins that regulate gene expression. HTH has two ? helices joined by a short strand of amino acids.Escherichia coli is seen to grow on fructoselysine. Fructoselysine is an Amadori adduct.It breaks down into furosine on acid-catalysed hydrolysis.E. coli breaks fructoselysin down using the enzymes fructoselysine-6-kinase. Next fructoselysine 6-phosphate deglycase into glucose 6-phosphate and lysine. Fructosamines (non-enzymatic) are the products of glucose.Expression:Caused by fructoselysine. Frlr Makes part of the frl operon. The frlr operon includes FrlA, FrlB, FrlC and FrlDBiological Process of the Frlr is transcription, DNA-templated, regulation of transcription, Molecular Function of Frlr is DNA binding, transcription factor activity, sequence-specific DNA binding Cellular Component: Located in the cytosol. Name of article: “Adaptive immunity increases the pace and predictability of evolutionary change in commensal gut bacteria”frlR codes for a predicted regulator of the fructoselysine operon The regulator of the fructoselysine operon is responsible for the metabolism of fructosamines.The mutations identified of frlr in the article may lead to inactivation of frlR.This causes the constitutive expression of the frl operon. Which means that the it is constantly active. This aviation would be beneficial to E. coli in the gut when nutrients are very low.